WebbBiotin is a water-soluble vitamin which functions as a coenzyme for carboxylases. Application For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation. Biotin-16-dUTP is used as asubstrate for: Terminal Transferase DNA polymerase I (holoenzyme and Klenow fragment) Taq DNA polymerase WebbI use 1.5-2 ug of DNA, 4 ul of dNTP mix (1mM dATP, dCTP, dGTP; 0.66 mM dTTP and 0.33 mM labeled-dUTP), 2 ul 10X buffer, H2O and 2 ul enzyme mix from Roche Nick …
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WebbDIG-Nick Translation Mix: 11745816910: 160 µl,40次标记反应: DIG-High Prime DNA Labeling and Detection Starter Kit I: 11745832910: 12次标记反应,24次杂交检测: DIG-High Prime DNA Labeling and Detection Starter Kit Ⅱ: 11585614910: 12次标记反应,24次杂交检测: PCR DIG Labeling Mix: 11585550910: 500ul,50次标记 ... Webb23 nov. 2014 · PCRproducts should HighPure PCR Product Purification Kit* before nick translation removeexcess unincorporated dNTPs. Step Action templateDNA sterile,double dis- tilled water endup finalvolume DIG-NickTranslation Mix, mix centrifugebriefly. 90min EDTA (pH 8.0) 10min. Step Action Afterincubation 15C,place aliquotper 20 … bomb tower clash
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WebbNick Translation Mix は、in situプローブの直接的な蛍光物質ラベリング用にデザインされています。 Roche Applied Science のフルオレセイン-12-dUTP およびテトラメチル … WebbOptimized enzyme mixture: Nick translation utilizes a combination of DNase and DNA Polymerase to nick one strand of the DNA helix, then incorporates labeled nucleotides … WebbFör 1 dag sedan · 8 Add 1 μl of a dNTP solution (each at 10 mM) and 2 μl (5 units) of bacteriophage T4 DNA polymerase. Incubate the reaction mixture at 15–25 °C for 15 … bomb tower paragon