Then plvx-tetone-3xf-n-term puro

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Splet21. jul. 2024 · pLVX-Puro慢病毒载体使用说明.pdf. 2024-07-21上传. pLVX-Puro慢病毒载体使用说明 Splet2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then …

An improved Tet-on system in microRNA overexpression and CRISPR/Cas9 …

Splet27. maj 2024 · 载体描述. pLVX-IRES-Puro is an HIV-1-based, lentiviral expression vector that allows the simultaneous expression of your protein of interest and puromycin resistance (Puror ) in virtually any mammalian cell type, including primary cells. The vector expresses your protein of interest and Puror from a bicistronic mRNA transcript, allowing ... SpletThe Lenti-X Tet-One Inducible Expression System (Puro) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in a wide variety of dividing and non-dividing … egyptian cotton sheets bed bath beyond

pLVX-TetOne-Puro Vector Map - Takara Bio

Splet16. sep. 2024 · NKTCLs are almost exclusively extranodal involving the nasal and/or paranasal area, with a strong association with Epstein–Barr virus (EBV) infection 3, 4, 6, 7, 8. Despite a multimodality... http://www.youbio.cn/product/vt8006 SpletThe pLVX-TetOne-Puro lentiviral vector enables transgene expression under the tight control of the TRE3G promoter that consists of seven repeats of a 19-bp tet operator … egyptian cotton sheets benefits

pLVX-Puro Vector Information - Addgene

SpletIn addition to lentiviral elements, pLVX-Puro contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (P. PGK. ) for … Splet06. nov. 2024 · SBI 第三代慢病毒载体 SBI offers thirdgeneration mostpopular HIV-1 based lentivector expression system which consists threemaincomponents: lentiviralexpression vector (e.g., pCDH-EF1-MCS-T2A-Puro) lentiviralpackaging plasmids (e.g., pPACKH1 Packaging Plasmid mix) pseudoviralparticle producer cell line (e.g., 293TN cells) 2A … egyptian cotton sheets 400 countSplet优宝生物提供pLVX-IRES-Puro-3xFlag载体,产品优质,服务快速,同时提供pLVX-IRES-Puro-3xFlag质粒图谱,载体序列,载体抗性及操作手册等信息 egyptian cotton sheets black friday sale

"SpletA good question, actually, there are pLVX-TetOne-EGFP-Puro vectors. For GFP, you need a cell sorter to get many pure GFP+ cells. For copGFP, I am wondering the harm of copGFP … " - Then plvx-tetone-3xf-n-term puro

Then plvx-tetone-3xf-n-term puro

SpletpLVX-TetOne-Puro (Search Vector Database) Backbone manufacturer Clontech Backbone size w/o insert (bp) 9227 Total vector size (bp) 11904 Vector type Mammalian … SpletThe pLVX-TetOne-Puro Vector Set, which is part of the Lenti-X Tet-One Inducible Expression System \(Puro\) allows for the lentiviral delivery and inducible expression of your gene of …

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SpletpLVX-TetOne-Puro Sequence and Map pLVX-TetOne-Puro All-in-one lentiviral vector with a puromycin resistance marker, for strong inducible expression of genes using the Tet-On® …

Splet31. maj 2024 · The pMD2.G and psPAX2 packaging plasmids and the pLVX-TetOne-Puro vector constructs for expressing ZNF24 or β-catenin overexpression vector were transfected into HEK293 cells to silence expression using Lipofectamine 2000 (Cat.No.11668019, Invitrogen) in accordance with specific protocols. ... Then, we chosen … Splet29. mar. 2024 · HH-LV-029 pLVX-TetOne-Puro调控应答一体四环素诱导型慢病毒载体嘌呤霉素 HH-LV-028 pLV-N-Flag-mCherry-IRES-Puro慢病毒过表达载体红色荧光 HH-LV-027 pLVX-Tet3G 慢病毒表达载体四环素诱导系统

SpletpLVX-IRES-Puro. Lentiviral vector for bicistronic expression of a gene together with a puromycin resistance marker. Explore Over 2.7k Plasmids: Viral Expression & Packaging … SpletWhen using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why …

Splet10. jun. 2024 · An artificially synthesized MCS-P PGK -TetON3G-P2A-Puro fragment was PCR-amplified with Pfu DNA Polymerase (Promega, Madison, WI, USA) and excised with Cla I, followed by ligation to the above recovered pLVX-Puro, generating pLVX-Tet2A-Puro.

Splet10. sep. 2024 · pLVX-TetOne-Puro 慢病毒载体；四环素调控载体 pLV-N-Flag-mCherry-IRES-Puro慢病毒过表达载体红色荧光 pLVX-Tet3G 慢病毒表达载体四环素诱导系统 pLVX-Tre3G 慢病毒表达载体四环素诱导系统 pLVX-DsRed-Monomer-N1 慢病毒红色荧光 Puro pVSV-G 第三代慢病毒包装质粒 pLP-VSVG 慢病毒包装载体 pLP2 第三代慢病毒包装质粒 pLP1 第三 … egyptian cotton sheets black fridaySpletpLVX-Puro. Lentiviral expression vector with the constitutive CMV promoter and a puromycin resistance marker. Sequence Author: Clontech (TaKaRa) Open in SnapGene. … folding scooter 350 lb weight capacitySpletpLVX-TetOne-Puro载体质粒图谱、序列、价格、抗性、测序引物、大小等基本信息_生物风载体 pLVX-TetOne-Puro 价格： 6500元联系方式： I47-825O-882O 相关技术服务：质粒构建基因合成质粒大提立刻购买买家导航载体基本信息载体质粒图谱和多克隆位点信息载体简介载体序列 egyptian cotton sheet sets queen sizeSpletpLVX-puro (Search Vector Database) Vector type Lentiviral Selectable markers Puromycin Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature … folding scooter electricSplet03. dec. 2024 · pLVX-TetOne-Puro. Catalog No. PVT11126 Packing 2ug . pLVX-TetOne-Puro Information. Prokaryotic resistance: ampicillin Amp. Screening markers: … folding scooter for 3 year oldSpletVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community … egyptian cotton sheets king deep pocketSpletThe all-in-one pLVX-TetOne-Puro Vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter in the forward orientation and your gene of interest … egyptian cotton sheet set cal king